Peer-reviewed Publications |
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Boudjemaa, R., Cabriel, C., Dubois-Brissonnet, F., Bourg, N., Dupuis, G., Gruss, A., Lévêque-Fort, S., Briandet, R., Fontaine-Aupart, M.-P., Steenkeste K. (2018). Failure of daptomycin to kill Staphylococcus aureus: impact of bacterial membrane fatty acid composition. Antimicrobial Agents and Chemotherapy, .
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Boudjemaa, R., Steenkeste, K., Jacqueline, C., Briandet, R., Caillon, J., Boutoille, D., Le Mabecque, V., Tattevin, P., Fontaine-Aupart, M.P., Revest, M. (2018). Live intramacrophagic Staphylococcus aureus as possible responsible for antibiotic therapy failure: observations in an in-vivo mouse model of prosthetic vascular material infections. Journal of Antimicrobial Chemotherapy, .
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Cabriel, C., Bourg, N., Dupuis, G., & Leveque-Fort, S. (2018). Aberration-accounting calibration for 3D single-molecule localization microscopy. Opt Lett, 43(2), 174–177.
Résumé: We propose a straightforward sample-based technique to calibrate the axial detection in 3D single-molecule localization microscopy. Using microspheres coated with fluorescent molecules, the calibration curves of point spread function-shaping or intensity-based measurements can be obtained over the imaging depth range. This experimental method takes into account the effect of the spherical aberration without requiring computational correction. We demonstrate its efficiency for astigmatic imaging in a 1.2 mum range above the coverslip.
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Kuncic, Z., & Lacombe, S. (2018). Nanoparticle radio-enhancement: principles, progress and application to cancer treatment. Physics In Medicine And Biology, 63(2).
Résumé: Enhancement of radiation effects by high-atomic number nanoparticles (NPs) has been increasingly studied for its potential to improve radiotherapeutic efficacy. The underlying principle of NP radio-enhancement is the potential to release copious electrons into a nanoscale volume, thereby amplifying radiation-induced biological damage. While the vast majority of studies to date have focused on gold nanoparticles with photon radiation, an increasing number of experimental, theoretical and simulation studies have explored opportunities offered by other NPs (e.g. gadolinium, platinum, iron oxide, hafnium) and other therapeutic radiation sources such as ion beams. It is thus of interest to the research community to consolidate findings from the different studies and summarise progress to date, as well as to identify strategies that offer promising opportunities for clinical translation. This is the purpose of this Topical Review.
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Liao, Y. Y., Melissen, S. T. A. G., Audibert, J. F., Vu, T. T., Clavier, G., Meallet-Renault, R., Retailleau, P., Lemaistre, J. P., Genot, V., & Pansu, R. (2018). Fluorescence Spectroscopy of AdamBODIPY Single Crystals. Chemphotochem, 2(2), 72–80.
Résumé: Interest in the fluorescence of organic solids is increasing with the development of nanosensors and research into new molecules with aggregation-induced fluorescence properties. We have gone beyond the qualitative observation of fluorescence by analyzing the luminescence properties of planar single crystals of a 4,4'-difluoro-4-bora-(3a,4a)-diaza-s-indacene (BODIPY) derivative with micrometric dimensions. A simple Frenkel exciton model applied to this crystal predicts one band. From time-resolved fluorescence spectra, three emissions were distinguished. The shortest-lived one at lambda = 547 nm was predicted by theory and corresponds to an exciton lifetime of 0.9 ns. A trap with an intermediate emission lifetime of 1.2 ns was found at 569 nm and a final trap at 620 nm has a lifetime of 1.9 ns. These attributions were confirmed by the study of the polarization of these emissions. The 547 nm emission was polarized along the long axis of the crystal as predicted by the Frenkel exciton model. The 569 nm emission was polarized perpendicularly to the plane of the crystal and the 620 nm emission was polarized along the short axis. Thus, the two red-shifted bands were related to well-defined defects with specific orientations in the crystal. Fluorescence lifetime imaging measurements showed that the density of these defects is not uniform and that under our synthesis conditions, they are formed in the initial steps of the growth and therefore appear in the center of the crystals.
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Mathieu, M. - C., Toullec, A., Benoit, C., Berry, R., Validire, P., Beaumel, P., Vincent, Y., Maroun, P., Vielh, P., Alchab, L., Farcy, R., Moniz-Koum, H., Fontaine-Aupart, M. - P., Delaloge, S., & Balleyguier, C. (2018). Preclinical ex vivo evaluation of the diagnostic performance of a new device for in situ label-free fluorescence spectral analysis of breast masses. European radiology, , 1–9.
Résumé: OBJECTIVES: To assess the diagnostic performance of a new device for in situ label-free fluorescence spectral analysis of breast masses in freshly removed surgical specimens, in preparation for its clinical development. METHODS: Sixty-four breast masses from consenting patients who had undergone either a lumpectomy or a mastectomy were included. Label-free fluorescence spectral acquisitions were obtained with a 25G fibre-containing needle inserted into the mass. Data from benign and malignant masses were compared to establish the most discriminating thresholds and measurement algorithms. Accuracy was verified using the bootstrap method. RESULTS: The final histological examination revealed 44 invasive carcinomas and 20 benign lesions. The maximum intensity of fluorescence signal was discriminant between benign and malignant masses (p < .0001) whatever their sizes. Statistical analysis indicated that choosing five random measurements per mass was the best compromise to obtain high sensitivity and high negative predictive value with the fewest measurements. Thus, malignant tumours were identified with a mean sensitivity, specificity, negative and positive predictive value of 98.8%, 85.4%, 97.2% and 93.5%, respectively. CONCLUSION: This new in situ tissue autofluorescence evaluation device allows accurate discrimination between benign and malignant breast masses and deserves clinical development. KEY POINTS: A new device allows in situ label-free fluorescence analysis of ex vivo breast masses Maximum fluorescence intensity discriminates benign from malignant masses (p < .0001) Five random measurements allow a high negative predictive value (97.2%).
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Actes de Conférences |
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Toullec, T., Mathieu, M. - C., Benoit, C., René Farcy, R., Tourasse, C., Boisserie-Lacroix, M., Fontaine-Aupart, M. - P., Delaloge, S., & Balleyguier, C. (2018). 25 gauge fibered-needle for label free fluorescence analysis of breast masses: a first in vivo study. In Proc.SPIE 10488, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications, (Vol. XVIII).
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