Peer-reviewed Publications |
Aubry, G., Meance, S., Couraud, L., Haghiri-Gosnet, A. - M., & Kou, Q. (2009). Intracavity microfluidic dye laser droplet absorption. MICROELECTRONIC ENGINEERING, 86(4-6), 1368–1370.
Résumé: An original design for droplet absorption measurement is presented. A microfluidic dye laser is combined with a droplet production system to realise intracavity absorption measurement on few nanoliter volumes. Conditions on droplets for obtaining laser signal are investigated and absorption measurement proof of concept is demonstrated. (C) 2009 Elsevier B.V. All rights reserved.
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Blandin, P., Leveque-Fort, S., Lecart, S., Cossec, J. C., Potier, M. - C., Lenkei, Z., Druon, F., & Georges, P. (2009). Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source. APPLIED OPTICS, 48(3), 553–559.
Résumé: We present the instrumental development of a versatile total internal reflection fluorescence lifetime imaging microscopy setup illuminated by a supercontinuum laser source. It enables performing wide-field fluorescence lifetime. imaging with subwavelength axial resolution for a large range of fluorophores. The short overall acquisition time and the axial resolution are well suited for dynamic neurobiological applications. (C) 2009 Optical Society of America
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Eschwege, P., Deniset-Besseau, A., Ferlicot, S., Lecart, S., Leveque-Fort, S., Lwaleed, B. A., Benoit, G., Droupy, S., Hubert, J., & Fontaine-Aupart, M. P. (2009). DETECTION OF HUMAN BLADDER CANCER CELL RESISTANCE TO MVAC CHEMOTHERAPY BY FLUORESCENCE IMAGING. EUROPEAN UROLOGY SUPPLEMENTS, 8(4), 152.
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Lacombe, S., & Le Sech, C. (2009). Advances in radiation biology: Radiosensitization in DNA and living cells. SURFACE SCIENCE, 603(10-12), 1953–1960.
Résumé: One fundamental goal of radiation biology is the evolution of concepts and methods for the elaboration of new approaches and protocols for the treatment of cancers. In this context, the use of fast ions as ionizing particles offers the advantage of optimizing cell killing inside the tumor whilst preserving the surrounding healthy tissues. One extremely promising strategy investigated recently is the addition of radiosensitizers in the targeted tissue. The optimization of radiotherapy with fast ions implies a multidisciplinary approach to ionizing radiation effects on complex living systems, ranging from studies on single molecules to investigations of entire organisms. In this article we review recent studies on ion induced damages in simple and complex biological systems, from DNA to living cells. The specific aspect of radiosensitization induced by metallic atoms is described. As a fundamental result, the addition of sensitizing compounds with ion irradiation may improve therapeutic index in cancer therapy. In conclusion, new perspectives are proposed based on the experience and contribution of different communities including Surface Sciences, to improve the development of radiation biology. (C) 2009 Elsevier B.V. All rights reserved.
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Le Moal, E., Fort, E., & Leveque-Fort, S. (2009). Metallic films and nanostructures for molecular fluorescence enhancement. ACTUALITE CHIMIQUE, (332), 36–44.
Résumé: Metallic films and nanostructures for molecular fluorescence enhancement Molecule fluorescence is subject to the influence of its direct electromagnetic environment. Excitation and emission processes can be modified by the presence of a metallic structure, due to interference phenomena and coupling to surface plasmon modes. Metallic films ranging in morphology from nanoparticles to percolated, continuous, plane and rough films were designed and characterized. Their influence on the optical behaviour of the fluorophores was investigated by experiments and a theoretical model, as a function of fluorophore-to-metal distance and molecular orientation. The detected signal is found to be amplified by one to two orders of magnitude. Moreover, fluorophore photostabilization and the modification of intermolecular energy transfer processes are reported. This paper demonstrates the interest in this technology for sensitivity improvement of DNA chip and for applications in cell and tissue imaging.
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Le Moal, E., Leveque-Fort, S., Potier, M. - C., & Fort, E. (2009). Nanoroughened plasmonic films for enhanced biosensing detection. Nanotechnology, 20(22), 225502.
Résumé: Although fluorescence is the prevailing labeling technique in biosensing applications, sensitivity improvement is still a striving challenge. We show that coating standard microscope slides with nanoroughened silver films provides a high fluorescence signal enhancement due to plasmonic interactions. As a proof of concept, we applied these films with tailored plasmonic properties to DNA microarrays. Using common optical scanning devices, we achieved signal amplifications of more than 40-fold.
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Parent, P., Bournel, F., Lasne, J., Lacombe, S., Strazzulla, G., Gardonio, S., Lizzit, S., Kappler, J. - P., Joly, L., Laffon, C., & Carniato, S. (2009). The irradiation of ammonia ice studied by near edge x-ray absorption spectroscopy. JOURNAL OF CHEMICAL PHYSICS, 131(15), 154308.
Résumé: A vapor-deposited NH(3) ice film irradiated at 20 K with 150 eV photons has been studied with near-edge x-ray absorption fine structure (NEXAFS) spectroscopy at the nitrogen K-edge. Irradiation leads to the formation of high amounts (12%) of molecular nitrogen N(2), whose concentration as a function of the absorbed energy has been quantified to 0.13 molecule/eV. The stability of N(2) in solid NH(3) has been also studied, showing that N(2) continuously desorbs between 20 and 95 K from the irradiated ammonia ice film. Weak concentrations (<1%) of other photoproducts are also detected. Our NEXAFS simulations show that these features own to NH(2), N(2)H(2), and N(3)(-).
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Actes de Conférences |
Ros, D., Kazamias, S., Guilbaud, O., Habib, J., Zielbauer, B., Pittman, M., Jamelot, G., Klisnick, A., Lagron, J. - C., Joyeux, D., de Rossi, S., Delmotte, F., Lacombe, S., Porcel, E., Lesech, C., Penhoat, A. M., & Touati, A. (2009). Recent Advances on LASERIX Facility: Development of XUV Sources System and Applications. Perspectives from 2008 to 2010. In X-RAY LASERS 2008 (Vol. 130, pp. 13–22).
Résumé: LASERIX is a high-power laser facility leading to High-repetition-rate XUV laser pumped by Titanium:Sapphire laser. The aim of this laser facility is to offer Soft XRLs in the 30-7 nm range and auxiliary IR beam that could also be used to produce synchronized XUV sources. This experimental configuration highly enhances the scientific opportunities of the facility, giving thus the opportunity to realize both X-ray laser experiments and more generally pump/probe experiments, mixing IR and XUV sources. In this contribution, the main results concerning both the development of XUV sources (in the seeded or ASE mode) and their use for applications (irradiation of DNA samples) are presented.
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Zielbauer, B., Habib, J., Kazamias, S., Guilbaud, O., Pittman, M., Ros, D., du Penhoat, M. - A. H., Touati, A., Le Sech, C., Porcel, E., & Lacombe, S. (2009). Strand Breaks in DNA Samples Induced with LASERIX. In X-RAY LASERS 2008 (Vol. 130, pp. 409–415).
Résumé: Samples of plasmid DNA were irradiated with pulsed 18.9 nm radiation originating from a Mo X-ray laser pumped in GRIP configuration at the LASERIX facility [1]. Surface doses of up to 500 kGray were delivered with a repetition rate of 10 Hz and average pulse energy of 200 nJ. The occurrence of single and double strand breaks not present in controls having undergone the same treatment except for the XRL irradiation can be seen as a clear effect of the XRL irradiation. This confirms the role of direct effects in DNA damages as previously seen with low energy ions [2, 3] and electrons.
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